RNA Extraction in Toxicology: A Comparative Analysis of High-Throughput Kits
RNA extraction and purification are essential tools in toxicology, providing valuable insights at the molecular level. Successfully extracting high-quality RNA is crucial for gene expression profiling, elucidating molecular mechanisms, identifying biomarkers, and developing targeted therapies to mitigate toxic effects.
Numerous RNA extraction kits options are available, each with its unique extraction chemistry. High-throughput kits are preferred in the industry due to their rapid sample processing, reduced hands-on time, minimized contamination risk, and increased reproducibility. However, this field has received little attention, resulting in a lack of standardized guidelines and consistency among commercially available kits, leading to significant variability. Consequently, researchers must navigate a complex landscape of extraction kits, making it challenging to establish best practices.
Our study aims to address these challenges by comparing high-throughput RNA extraction kits using naïve non-human primate (NHP) tissues. We highlight the strengths and limitations of current extraction methods, providing a framework for best practices. By facilitating method selection, we aim to establish a baseline for future discussions on RNA extraction. Ultimately, our findings seek to enhance the reliability and reproducibility of gene and cell therapy research, paving the way for improved protocols in toxicological studies.
Read our recent publication and learn more at www.lovelacebiomedical.org.